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- roche
- 罗氏 10650129001
- 2025年11月14日
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- 详细信息
- 技术资料
- 保质期：
见产品外包装
- 英文名：
Restriction Endonuclease Pvu I
- 库存：
需确认
- 供应商：
嵘崴达
- CAS号：
见产品外包装
- 保存条件：
−20°C
- 规格：
500 UNITS
Pvu I
from Proteus vulgaris
别名:
restriction enzyme
特异性
Pvu I recognizes the sequence CG°AT↓ *CG and generates fragments with 3′-cohesive termini.
Recognition sites: CG°AT*CG
CG°AT*CG
Restriction site: CG°AT↓*CG
CG°AT↓*CG
Heat inactivation: No inactivation of Pvu I after incubation at 65 °C for 15 minutes.
质量
Absence of nonspecific endonuclease activities
1μg λDNA is incubated for 16hours in 50μl SuRE/Cut Buffer H with an excess of Pvu I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5μg [3H] labeled calf thymus DNA are incubated with 3μl Pvu I for 4hours at +37°C in a total volume of 100μl 50mM Tris-HCl, 10mM MgCl2, 1mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
DNA图谱分析
Number of cleavage sites on different DNAs
- λ: 3
- φX174: 0
- Ad2: 7
- M13mp7: 1
- pBR322: 1
- pBR328: 1
- pUC18: 2
- SV40: 0
| 10650129001 | Restr.-Endonucl. Pvu I, 500 U | 500 U (5 U/µl) | |
| 10667145001 | Restr.-Endonucl. Eco RV, 2000 | 2,000 U (10 U/µl) | |
| 10674273001 | Restr.-Endonucl. Xba I, 20 000 | 20,000 U (10 U/µl) | |
| 10776777001 | Restr.-Endonucl. Nru I, 1000 u | 1,000 U (10 U/µl) | |
| 10822230001 | Restr.-Endonucl. Mae III, 50 u | 50 U (1 - 5 U/µl) | |
| 10885843001 | Restr.-Endonucl. Nhe I, 200 u | 200 U (10 U/µl) | |
| 10899194001 | Restr.-Endonucl. Xho I, 5000 U | 5,000 U (10 U/µl) | |
| 11008951001 | Restr.-Endonucl. Spe I | 1,000 U (10 U/µl) | |
| 11026534001 | Restr.-Endonucl. Sph I, 2500 U | 2,500 U (10 U/µl) |
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