ER-TRACKER(TM) BLUE-WHITE

Cloning PCR products using TA vectors

has been found to give high white-to-blue-ratio background problems in the absence of added insert, while the pCR [TM] II has a replication region of pBR322 and a relatively low copy number compared to the others of pUC origin, which can be a disadvantage. Some suggested

Cloning PCR products using TA vectors

for this, including the pBluescript derivative pCR-Script [TM] SK(+) cloning kit from Stratagene, pCR [TM] II supplied with the TA Cloning [TM] kit from Invitrogen, pT7Blue T-Vector kit from Novagen, pGEM[R]-T from Promega, and the SureClone [TM] ligation kit

Optimized Welsh DDRT

formamide, 10mM , pH 8.0, 0.025% bromophenol blue and 0.025% xylene cyanol), acrylamide, bis-acrylamide, urea (molecular biology grade), Whatmann 3MM paper , Tracker Tape TM (Amersham International), X-Ray film s. Ethanol , Na Acetate . TA-Cloning Kit