CLICK-IT PROTEIN REACTION BUFF

Synthesis of Alkyne‐ and Azide‐Modified Oligonucleotides and Their Cyclization by the CuAAC (Click) Reaction

  Figure 4.33.5 Gel electrophoresis analysis of hairpin loops (H; purified) and the click reaction mixture (C) on a 20% polyacrylamide/7 M urea gel 3 hr at a constant power of 20 W, using 0.09 M TBE buffer, pH

The Polymerase Chain Reaction (PCR)

Topics　　• What is the Polymerase Chain Reaction?　　• History and (pre-history) of PCR　　• How PCR works　　• Optimising PCR　　• Fidelity, errors and cloning　　• PCR primer design　　• Applications of PCR What is the Polymerase Chain Reaction?　　• It’s

Conjugation of Ligands to the Surface of Preformed Liposomes by Click Chemistry

Click chemistry represents a new bioconjugation strategy that can be used to conveniently attach various ligands to the surface of preformed liposomes. This efficient and chemoselective reaction involves a Cu(I)-catalyzed azide-alkyne