MEDIUM 200 500ML

BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method

antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells

BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod

is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets

血液基因组 DNA提取试剂盒操作指南（DP348） ——200μl~1ml 抗凝全血

以下操作按照天根产品 DP348 血液基因组 DNA 提取试剂盒的说明书进行，所有反应现象仅适用于该产品及本文所标明的样本量。如使用其它操作流程或产品，样本量不同可能现象与本文有所差异。实验准备：1. 抗凝血 200 μl~1ml抗凝全血2. 移液器及配套无菌枪头（200 μl ，1ml）3. 无水乙醇4. 涡旋振荡器，金属浴/水浴，台式离心机备注：本实验以人血为例，如提取哺乳动物全血可以用此流程，如果禽类、鸟类、两栖类或更低级生物的抗凝血液，红细胞有核细胞，因此处理量为5-20 μl，当处理