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- invitrogen
- SA10004
- 2025年07月14日
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文献和实验Analysis of Protein-DNA Binding by Streptavidin-Agarose Pulldown
. We describe here a streptavidin-agarose pulldown assay that is capable of analyzing quantitatively binding of an array of proteins to DNA probes. The assay is easy to perform and does not require radiolabeled probes. It involves incubation of nuclear extract proteins
Materials: agarose solution in TBE or TAE (generally 0.7-1%) 1X TBE or TAE (same buffer as in agarose) gel loading dye
Colloidal Gold/Streptavidin Methods
. This nonspecific binding has been attributed both to its high isoelectric point (PI = 10) and to the fact that the protein is glycosylated (3 , 4 ). Because of the problem of nonspecific binding of avidin, streptavidin has largely replaced avidin
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