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- 17104019
- 2025年07月13日
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文献和实验Isolation of Bacterial Type IV Machine Subassemblies
The bacterial type IV secretion systems (T4SSs) deliver DNA and protein substrates to bacterial and eukaryotic target cells generally by a mechanism requiring direct contact between donor and target cells. Recent advances in defining
Immunoassays for Collagenase-Mediated Cleavage of Type I and II Collagens
This chapter describes the production and characterization of antibodies raised against neoepitopes in collagenase-cleaved collagen. It also details the development, validation, and use of immunoassays using such antibodies to measure
the Dot/Icm type IV secretion system which translocates a plethora of effectors into host cells. Determining whether a particular protein is a substrate of the transporter is a prerequisite for subsequent functional studies. Thus, a variety of methods
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