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- invitrogen
- 12648010
- 2025年07月13日
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文献和实验Cell Freezing Reagents / Solutions Cells! (at least 5 x 106 ). Foetal calf serum (FCS), heat inactivated at 65 � for 1 hr. Dimethylsulfoxide (DMSO). Freezing vials. Hi - Tech Cell
Human multipotential stromal cells (hMSCs) are easily isolated from bone marrow and can be expanded by up to 200-fold in culture. Cultures of hMSCs are heterogeneous mixtures of stem/progenitor cells and more mature cell types. The proportion
Cell Thawing/Cell Freezing Protocol
Freezing Cells : Cells should be growing well or known to be in log phase Count, collect and pellet cells in a 15mL test tube Resuspend in freezing media so that the concentration is no more than 5x10^6 cells
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