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- 2025年07月12日
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文献和实验Display and Selection of scFv Antibodies on HEK-293T Cells
We describe a human cell display strategy to isolate high-affinity single-chain antibody fragments (scFvs) specific for CD22 for the treatment of B-cell malignancies. Our strategy uses flow cytometry and human embryonic kidney 293T (HEK-293T
2 ) and incubate at 37°C until cells have detached (1-2 minutes). Prepare a new flask with fresh medium. Block trypsinization by adding a few ml of medium. Take a fraction of the cell solution and inocculate the new flask. Typically, when splitting confluent HEK
Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays
-well microtitre plate to form an array. These vectors are then packaged in lipid-based transfection reagent, cells grown over the top of the array are transfected and the arrays can then be examined for alterations in cellular function as manifested in localised
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