AmpFLSTR Yfiler PCR Amplificat

AllPrep DNA/RNA 96 Kit

using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (-RT) indicates

FFPE Direct PCR Kit

for 60 minutes.    5) Add 100 μl FP3 Buffer and vortex to mix.    6) Store the extraction at 2-8°C. 2. PCR Protocol This protocol serves as a guideline for PCR amplification. Optimal reaction conditions, such as incubation times

SequalPrep Long PCR Kit with dNTPs

μl of master mix into each PCR tube/well, and add: Primer mix (10 μM each)              1 μl           0.5 μM each Template DNA (1–100 ng/μl)          1 μl             as required 4.        Cap the tube/well, tap