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货号:4462907
产品名称:ION FRAGMENT LIBRARY KIT
品牌:abi
关键字:abi,Applied Biosystems公司,4462907,ION FRAGMENT LIBRARY KIT
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
) (see Step 39) The kit includes end-repair enzyme mix, end-repair 10X buffer, dNTP solution, and ATP. Dynabeads Protein A (Invitrogen) Ethanol (70% and 100%) Glycerol Glycogen, 20 mg/mL Klenow fragment exo- (optional; see Step
Preparation of a Phage DNA Fragment Library for Whole Genome Shotgun Sequencing
The most efficient method to determine the genomic sequence of a dsDNA phage is to use a whole genome shotgun approach (WGSA). Preparation of a library where each genomic fragment has an equal chance of being represented
by a two- or three-step procedure. The method can insert any fragment into small or large episomes, without the need of an antibiotic selection gene. We have humanized the mouse luteinizing hormone receptor gene (Lhcgr ) by inserting a ~55 kb fragment from a BAC clone
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