PREPFILER EXPRESS F DNA EXT KI

乙醇固定样品DNA 抽提和直接PCR

管置于90℃水浴或者PCR 仪上5min，稍微冷却后加入50ul AT3 涡旋混匀。     5) 以上裂解液就可以作为PCR 的模板直接使用，PCR 体系中建议加入5ul 左右。     6) 取5ul E.Z.N.A. Tissue Direct PCR kit 抽提得到的一条鱼苗的DNA 作为模板，客户提供的引物Y-30-R/F 作为引物，PCR 扩增。     7) PCR 扩增程序：         94℃ 2min；      

Isolation of Retroelement from Plant Genomic DNA

      The amplification of DNA fragments using the polymerase chain reaction (33) is performed in either the Perkin-Elmer Cetus DNA Thermal Cycler or the Perkin-Elmer Cetus Cycler 9600, by adding the following reagents

siRNA设计指南

for directional cloning into this vector. The loop sequence and length can be varied as desired.For preparing SECs containing an H1 or U6 promoter by PCR using the Silencer Express Kits, (1) one or two DNA oligonucleotides encoding the siRNA sequence are designed