OBS-FG,FMAT GaM IgG BEADS

Purification of mAb (IgG)

(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10

Purification of mAb (IgG)

do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10 the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody

干货| CUT and Tag 技术全方位解答

&Tag 技术混淆，CUT&Tag 技术在异染色质位点上有很好的结果，Henikoff 推荐的阳性对照就是存在于异染色质区域的 H3K27me3，且我们的客户在 H3K27me3 以及 H3K27AC 上都有很好的结果。 4. ConA beads 的作用是什么？如果没有 ConA beads ，能否用离心法代替？ ConA beads 是经过 Concanavalin A（刀豆蛋白A，ConA）包被的磁珠。ConA 是一种从刀豆和豌豆等籽粒中提取出来的植物