GENEAMP XL PCR KIT (50 RXN)

Plant Seed Direct PCR Kit

is covered by the Extraction Solution.    2) Incubate at 56°C for 1 hours.    3) Incubate at 95°C for 5 minutes.    4) Add 50 -100 μl PS3 Buffer and vortex to mix.    5) Store the extraction at 2-8°C. 3. PCR Protocol

PCR/RT-PCR指南---PCR产物克隆

PCR Cloning Kit）（Topo XL PCR Cloning Kit） 因为越来越多高确限度热稳定酶如Pfx、Pfu被用于PCR 扩增，使到PCR 后都只是平端产物。这种平端克隆 往往效率低，并且有非特异性背景菌落产生，造成筛选困难。 Topo平端克隆 主要改良载体设计，把ccdB （Control of cell death）基因并入到mcs中，如果没有DNA片断插入，ccdB基因则会表达

Miniprep/Qiagen kit

  Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.