RGNT,DNA FRAGMENT ANALYSIS

RFLP Method - Restriction Fragment Length Polymorphism

Therefore, when Jack and Jill have their DNA subject to RFLP analysis, they will have one band in common and one band that does not match the other"s in molecular weight:

DNA Fragment Purification

2.Frozen agarose gel piece containing the desired DNA fragment Supplies: 1.Micropipetter and tips 2.Microcentrifuge and tubes 3.Spatula 4.PCR machine and the 600-ul tubes for use in the machine 5.Ultrafree-MC

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

in the design of targeting vectors and the subsequent functional analysis of newly discovered genes. Manipulation of plasmid DNA by homologous recombination was first reported in yeast (9) and since then techniques have been developed to include phage