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- abi
- 4426503
- 2025年06月30日
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货号：4426503
产品名称：SAMPLE LOOP,100ul PEEK
品牌：abi
关键字：abi，Applied Biosystems公司，4426503，SAMPLE LOOP,100ul PEEK
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文献和实验E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
tubes or microplates 实验步骤 1. Add 1-100 ul blood sample to a nuclease-free microcentrifuge tube. Bring the volume up to 100 ul with Elution Buffer provided with this kit. 2. Add 100 ul Buffer MSL and 10 ul Proteinase K
phenol / 8-OH-quinoline and vortex, add 100ul chloroform / isoamyl alcohol (24:1), vortex, spin: agarose should be in the phenol/CHCl3 phase. ALTERNATIVELY: take off mineral oil using 50ul CHCl3, take out plug of solidified sample and wash in TE
cycle, and load the sample into wells of a gel BEFORE submerging the gel: sample will set in the wells and not float out. 9. If you wish to extract DNA, end at 72oC and add 50ul pre-warmed phenol / 8-OH-quinoline and vortex, add 100ul chloroform
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