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- abi
- N8016112
- 2025年07月13日
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货号：N8016112
产品名称：FILTER-250UL INTEGRITY W/TIP
品牌：abi
关键字：abi，Applied Biosystems公司，N8016112，FILTER-250UL INTEGRITY W/TIP
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Inc.) per well. Invert gently several times during a 5 minute room temperature incubation. Let stand at room temperature for 1 minute before transferring to filter plate. 7. Using a wide bore pipette tip, transfer the lysate to a Qiagen TurboFilter™ (Qiagen
(72 °C, 5 min.) store PCR reactions at -20 °C until the gelrun IV. denaturing gel prepare 6 % denaturing polyacrylamide gel (7 M urea, 1 x TBE), prerun for 30 min at 60 W mix 3.5 µl of the PCR reaction with 2 µl
. Dissolve the precipitate in 100 x X ul dH2O (or 1/10 x TE) containing 1/10 x X ul RNase-It (Stratagene). Estimate the DNA concentration (by agarose gel electrophoresis with Etidium bromide or by Hoefer Dyna Quant 200) and make a solution adjusted at 100
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