SYRINGE, 250MIC L HAMILTON

Purification of recombinant sBRF M166L

. Filter the diluted sample through a 0.22 micron syringe filter before loading. Load the filtered sample to a 0.5 ml SOURCE 15 Q column equilibrated in B+ 50 mM KCl at a flow rate of 0.5 ml/min. Wash the column with 6 column volumes of B+ 50 mM KCl

Purification of recombinant sBRF M166L

with buffer B +0 KCl to a final KCl concentration of 50 mM.Filter the diluted sample through a 0.22 micron syringe filter before loading. Load the filtered sample to a 0.5 ml SOURCE 15 Q column equilibrated in B + 50 mM KCl at a flow rate of 0.5 ml/min. Wash

几种测定抗菌药物最低抑菌浓度（MIC）方法

生理性浓集的部位（如尿液）被抑制。另外，中介还作为“缓冲域”，以防止由微小的技术因素失控，所导致较大的错误解释。 1.2.微量肉汤稀释法 1.2.1.抗菌药物和培养基制备 同常量肉汤稀释法。 1.2.2.MIC板制备 无菌操作，将倍比稀释后不同浓度的抗菌药物溶液分别加到灭菌的96孔聚苯乙烯板中，第1至第11孔加药液，每孔10μl，第12孔不加药作为生长对照，冰冻干燥后密封，-20℃以下保存备用。 1.2.3.接种物制备 将用生长法或直接菌悬液法制备的浓度相当于0.5麦氏比浊标准