384-WELL CALIBRATION PLATE W/

Sequencing Reaction Cleanup in 384 Well Sequencing Reaction Plates

1.Take the 384 well viper plate from the thermocycler. Remove the Silicone Sealing Mat (Axygen Scientific: AM-384-PCR-RD) and place face down on a paper towel and pat to absorb any liquid that may be on it. Add 30 μl 95% EtOH/0.12 M NaOAc

【求助】抗体制备服务

in order to obtain a 620 nm signal within the 30,000-50,000 count range. This calibration was run in a 384-well low volume plate format using a final volume of 20 μL, a buffer containing 0.1% BSA and 0.4M KF, and a RUBYstar reader (BMG LABTECH

DNA测序实验方法步骤

are adjusted. Typically electrophoresis and data collection are for 10 hours at 30W on the ABI 373A that is fitted with a heat-distributing aluminum plate in contact with the outer glass gel plate in the region between the laser stop and the sample loading