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- 2026年01月05日
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货号：400987
产品名称：GEL TUBE 5PK,3.5x200mm
品牌：abi
关键字：abi，Applied Biosystems公司，400987，GEL TUBE 5PK,3.5x200mm
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文献和实验Single tube confirmation PCR protocol
(final elongation) 5. Agarose gel electrophoresis - Add 10 µl 6x loading buffer (30% glycerol, 50mM EDTA, 0.25% bromophenol blue) to the 50 µl PCR reaction. - Load 10 µl on a 1.5% agarose gel.
SDS Gel Electrophoresis of Tubulin\MAPs
% TCA Procedure Prepare 15 cm glass tubes with 6 mm internal diameter. Polymerize a 10 cm separation gel and a 1 cm stacking gel within the tubes by the addition of TEMED and ammonium persulfate as directed in Chapter Four.
Single tube confirmation PCR protocol
electrophoresis - Add 10 µl 6x loading buffer (30% glycerol, 50mM EDTA, 0.25% bromophenol blue) to the 50 µl PCR reaction. - Load 10 µl on a 1.5% agarose gel.
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