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- 2025年11月22日
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货号：4449755
产品名称：ATTUNE WASH SOLUTION
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文献和实验b-galactosidase Solution Assay
.1 g up to 500 ml 50 mM CPRG 0.0304 g chlorophenyl red b-galactopyranoside (Boehringer Mannheim #834-308) up to 1 ml with Q store in the dark at -20° Procedure • Wash cells or tissue twice in PBS, pellet and resuspend in 150 microliters
b-galactosidase Solution Assay
b-galactosidase Solution Assay Reference: Simon and Lis (1987) NAR 15 : 2971-2988. Solutions CPRG Assay Buffer 50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.5 1 mM MgCl2 0.1 ml 1M MgCl 2 up to 100 ml with Q
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
(Vector Laboratories PK 6100) (optional, see Step 30) Wash solution 1 Wash solution 2 Equipment Aluminum foil Diamond pen Eye protection (see Step 21) Filter paper (e.g., Whatman 3MM) Humid chamber for slide
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