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- abi
- 264-0001
- 2025年11月20日
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货号：264-0001
产品名称：GEAR,SPUR, 48OP,21T,.250 BORE
品牌：abi
关键字：abi，Applied Biosystems公司，264-0001，GEAR,SPUR, 48OP,21T,.250 BORE
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
culture fluid, inoculate a single or multiple 96 deep-well blocks containing 1.2-1.6 ml per well of LB media with appropriate antibiotic. Alternatively, 24 deep-well or 48 deep-well blocks may be used with up to 4.8 or 2.4ml of media respectively. Cover
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
colony, grow overnight at 370C. Using 3 ul of (overnight growth) single colony culture fluid, inoculate a single or multiple 96 deep-well blocks containing 1.2-1.6 ml per well of LB media with appropriate antibiotic. Alternatively, 24 deep-well or 48 deep
Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.
of overnight culture to inoculate 500ml of LB (+antibiotic) x 2 for a total culture volume of 1L, shake overnight (about 16hrs) at 37°C. 3. Collect cells by centrifugation in 250ml x 4 Nalgene bottles, (H-6000A rotor, 3800 rpm), 10 minutes, 4°C.
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