Firefly Luciferase GL2/3 siRNA

Construction of Simple and Efficient siRNA Validation Systems for Screening and Identification of Effective RNAi-Targeted Sequences from Mammalian Gen

. The efficiency of siRNAs is determined by their abilities to inhibit expression of the targeting reporters with easily quantified readouts including enhanced green fluorescence protein and firefly luciferase. Since only a readily available short synthetic DNA

哺乳动物RNAi技术 Mammalian RNA Interference

or to use a siRNA duplex that targets a gene absent from the selected model organism, e.g., GFP or luciferase. We have used an siRNA duplex targeting firefly luciferase as a control for targeting endogenous genes in mammalian cells because the firefly

v468.Chapter 8 采用 b-Catenin/TCF基本转录通讯结构对b-Catenin/TCF转录进行分析

& Glo ? reagent (Renilla luciferase reagentand Firefly luciferase antagonist). 3. Methods 3.1. Transient Transfection of Reporter for cDNA Overexpression or siRNA Knockdown Prior to the BAR system, the majority of Wnt