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- AM8860G
- 2025年08月24日
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货号：AM8860G
产品名称：3X miRNA Hybridization Buffer 2 ml x 5
品牌：abi
关键字：abi，Applied Biosystems公司，AM8860G，3X miRNA Hybridization Buffer 2 ml x 5
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文献和实验Clonality - X Chromosome Inactivation Assay
can be utilized. 1. Materials DNA sample (see Processing of Microdissected Tissue - DNA-based Analysis) Proteinase K (Sigma) Proteinase K buffer (0.05 M Tris-HCL, 0.001 M EDTA, 1% Tween 20, 0.1 mg/ml proteinase K, pH 8.0) Phenol:chloroform:isoamyl alcohol
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
RNA Purification Protocol for 1-2 x 107 cultured cells
设备 1. Microcentrifuge capable of 3,000 x g 2. 15 ml centrifuge tube 3. Ice bath 4. Ground pestle 实验步骤 1. Remove the media and collect cells. For cells grown in suspension, pellet the cell
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