MEGAclear Kit 20 rxns

Miniprep/Qiagen kit

from the handbook and annotated based on experience with the kit. Protocol: QIAprep Spin Miniprep Kit Using a Microcentrifuge This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1�5 ml overnight cultures of E. coli in LB

Human DC Enrichment Kit

volume of 35 ml at room temperature. 2)        Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3)        Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4)        Remove 20 ml of supernatant

MicroElute DNA Clean-Up Kit

column into a clean 1.5 mL micro centrifuge tube.    10) Add 15-20 ul Elution Buffer(10mM Tris, pH 8.5) or water to the center of the membrane, incubate at room temperature for 3-5 minute.    11) Centrifuge at maximal speed ($13,000 x g