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- abi
- AM7120G
- 2025年08月21日
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货号：AM7120G
产品名称：Yeast RNA (5 mg/ml) 500 ul (Shared)
品牌：abi
关键字：abi，Applied Biosystems公司，AM7120G，Yeast RNA (5 mg/ml) 500 ul (Shared)
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文献和实验RNA Isolation - Volumes and weights are for 10 ml cultures (1-2 x 107 cells/ml).1.Spin down cells, decant, and resuspend in 0.2 ml extraction buffer with SDS and transfer to a 1.5 ml eppendorf tube. The cells can be frozen at 70℃ at this point
In situ hybridization of yeast cells (RNA and Oligonucleotide probes)
is adapted from Long RM, et al. RNA 1995 Dec;1(10) :1787-1794. II. Fixation: Yeast are grown in 45ml cultures in the appropriate media until they reach early log phase (OD600 between 0.2 and 0.4). Cells are fixed for 10 minutes at room
sorbitol buffer. The cells can be stored at this point overnight at 4¡C. Zymolyase treat the cells with 30 ul 10 mg/ml Zymolyase (100T) at 30¡C for anywhere between 10-30 min or more. Examine cells on a phase microscope. When cells are dark
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