Coupling Buffer 400 ul

Coupling Peptides To Resin For Affinity Purification

. The amino resin will react with an NHS ester, whereas the original affigel and the iodoacetate will not. Take an aliquot (50ul) of resin at each step. Resuspend in 100 ul of buffer. Add 1 ul of 0.1 M NHS fluoroscein or NHS-rhodamine in DMSO. Incubate 5' RT

Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)

after the lysate is cleared. 8. Remove the plate containing the magnetic particles from the magnetic separation device. Add 400 ul of SPR Wash Buffer to each well and resuspend magnetic particles pellet by pipetting up and down or vortexing for 30 seconds

【求助】提质粒后，跑电泳，用10乘Loading Buffer 还是6乘Loading Buffer？

ilovelc999 请问各位，提质粒后，用20微升TE溶解，然后进行电泳，应该用10乘Loading Buffer 还是用6乘Loading Buffer？另外Buffer与质粒溶液一般应以什么比例混合好呢？多谢各位啦！ 纯属菜鸟 10乘 5：1的比例 质粒5 肥宝 我是用6X的，1:5，质粒5，不过也没那么严格，一般也就点个小点估计1ul lihuijin017