Figure 1: DetergentOUT™ GBS10 removes detergent & allows detection of peptide fragments by mass spectrometry. 500µg phosphorylase B was digested in solution & then the indicated amount of detergent was added. Samples were treated with DetergentOUT™ GBS10. Number of peptide spectra were determined as per the protocol of Alvarez, S. et al.
A. No detergent, No DetergentOUT™ GBS10 B. 0.5% CHAPS, DetergentOUT™ GBS10 treated
C. 0.5% CHAPS, No DetergentOUT™ GBS10
Figure 2: DetergentOUT™ GBS10 enhances mass spectrometryspectra. 5µg/µl protein mixture (BSA, cyctochrome C and phosphorylase B) in water (Panel A) was supplemented with 0.5% CHAPS (Panel B and C). The CHAPS containing sample was treated with DetergentOUT™ GBS10 and compared to an untreated sample (Panel C). Spectra were generated per Alvarez et al.
Table 1: A comparison of the detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10.
Figure 3: DetergentOUT™ GBS10 retains ≤6mg SDS per ml settled resin. SDS solution was continuously applied to DetergentOUT™ GBS10 column. The graph depicts the amount of SDS detected in the flow- through. SDS was not detected until fraction 7, so after 12mg SDS had been retained by the 2ml of DetergentOUT™ GB-S10 resin, resulting in a 6mg/ml settled resin binding capacity.
参考文献:
1. Alvarez, S. et al (2010) Poster presented as part of the 58th ASMS Conference on Mass
2. Spectrometry and Allied Topics, May 23-27, 2010, Salt Lake City, Utah
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6. Fisher, J. and Margulies, S. (2002) Am. J. Physiol. Lung Cell Mol. Physiol. 283: L737
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