ZINQUIN ETHYL ESTER 52020

Zinquin是UV激发的蓝色荧光锌指示剂。Zinquin ethyl ester是膜透性染料，进入细胞后被水解成Zinquin游离酸。锌被认为参与细胞凋亡抑制活动中，并且在许多神经活动中起到重要作用。

Zinquin is an UV-excitable, blue fluorescent zinc indicator. Zinquin ethyl ester is membrane-permeable and is hydrolyzed into Zinquin free acid once entering cells. Zinc is believed to be involved in the suppression of apoptosis and thought to play important roles in many neural activities.

• λEx/λEm (with Zn²⁺)= 364/485 nm (after hydrolysis)
• Off-white solid soluble in DMSO
• Store at 4°C and protect from light
• C₂₁H₂₂N₂O₅S
• MW: 414.48.
• [181530-09-6]

As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed. The organic anion transporter inhibitor Probenecid (#50027) can be used to slow the rate of indicator efflux from cells.