FURAPTRA,AM ESTER,100UG 50037

Furaptra的膜透性形式是Fura-2,AM ester。指示剂通过将细胞在含染料的缓冲液中与样品孵育进入细胞。

Furaptra, AM ester is a membrane-permeant form of Furaptra (Mag-Fura-2). The indicator is loaded into cells via simple incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic® F-127 (cat# 59004) is often used as a dispersing agent to facilitate cell loading. See information for Mag-Fura-2, tetrapotassium salt (cat# 50035).

• Light yellow solid soluble in DMSO
• Store at -20°C and protect from light
• C₃₀H₃₀N₂O₁₉
• MW: 723

BAPTA-based ion indicators like Furaptra have been shown to be fixable in situ by EDC/EDAC (cat# 59002). The fixation of indicator dyes is useful for downstream immunofluorescence and IHC studies (Cell Calcium 1997, 21(3), 175).

As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed. The organic anion transporter inhibitor Probenecid (#50027) can be used to slow the rate of indicator efflux from cells.