DiO/DPA膜电位检测试剂盒 30037

试剂盒包含一管绿色荧光膜染料DiOC16(3)（溶于DMSO）,一管阴离子膜淬灭染料DPA（溶于DMSO）。DiO/DPA系统利用荧光共振能量转移（FRET）原理检测细胞质膜电位变化，DiO 是FRET中的供体，而DPA作为FRET的受体。试剂盒中的DiOC16(3)是基于其他供应商的DiOC16(3)的改良产品。

DiO/DPA is a non-invasive optical membrane potential reporter system with low cytotoxicity, high speed, and high sensitivity.

Features

• Simple, non-invasive optical membrane potential monitoring
• Rapid, large fluorescence voltage response on submillisecond time scale
• Improved DiO formulation for better solubility

Kit Components

• DiOC₁₆(3) (DiO), 2 mM in DMSO, 1 mL
• Dipicrylamine (DPA), 20 mM in DMSO, 1 mL

The DiO/DPA system detects cytoplasmic membrane potential changes using the principle of fluorescence resonance energy transfer (FRET). The green fluorescent lipophilic dye DiO acts a stationary FRET donor in the plasma membrane. The anionic quencher DPA acts as a mobile FRET acceptor, rapidly partitioning in and out of the membrane in response to membrane potential, thus modulating DiO fluorescence. The DiO/DPA system has been reported to produce large fluorescence signal changes of >56% in HEK-293 cells and >25% in neuronal cultures and brain slices per 100 mV membrane potential change, and to report membrane potential in situ on submillisecond time scale (see Ref. 1).

The DiOC₁₆(3) in this kit is an improved version over the DiOC₁₆(3) from other suppliers; it is formulated with gluconate as the counter ion to increase dye solubility, for brighter and more uniform cell staining.

DPA is a hazardous material due to its explosive nature. To improve its safety during shipping and handling, it is provided as a dilute solution in the kit. We also offer DPA solid (catalog no. 60037).