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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
室温
- 英文名:
PLURONIC F-127 20% SOLUTION IN DMSO 59004
- 库存:
99
- 供应商:
Biotium
- CAS号:
9003-11-6
Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as cell-permeable AM esters of Fura-2, Fluo-3, Indo-1, or Rhod-2 calcium indicators to facilitate cell loading.
We also offer Pluronic® F-127 solid and Pluronic® F-127, 10% in H2O.
Pluronic F-127 is a registered trade mark of BASF.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验2+、Mg2+)洗2遍。加入Indo-1 AM(终浓度1~5μmol/L),37℃孵育30~60min。用PBS(含Ca22+、Mg2+)洗2遍。激光扫描共聚焦显微镜观察。 也可用弱的去垢剂Pluronic F-127助染。可将Pluronic F-127溶于DMSO配成20%(W/V)的溶液,终浓度1%~2%。 (四)Fluo-3测定细胞内Ca2+ 1. 储存液配制 Fluo-3(分子量为855),活细胞标记常选用Fluo-3 AM,分子量为1130,呈橙色粉状,避光冷藏保存或将其溶于
Culture of BEND Cells (Bovine Endometrial Cells)
to step 8. 2. Using hemacytometer, calculate cell number. 3. Dilute cells to concentration of 2 million cells per ml of medium. 4. Slowly drip in equal volume of 20% (v/v) DMSO solution in culture medium 5. Allocate cells in 1 ml cryotubes
Culture of BEND Cells (Bovine Endometrial Cells)
in equal volume of 20% (v/v) DMSO solution in culture medium 5. Allocate cells in 1 ml cryotubes. On each tube make note of date name and passage number. 6. Place at -70 o C for 24 hours. 7. Store cells in LN2. 8. Record storage location, passage












