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- Biotium
- 美国
- 10016
- 2026年01月22日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 英文名：
X-GLCU, CHA (5-BROMO-4-CHLORO-3-INDOLYL- 10016
- 库存：
99
- 供应商：
Biotium
- CAS号：
18656-96-7
X-GlcU (5-Bromo-4-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt) is a chromogenic β-glucuronidase substrate widely used for monitoring E. coli contamination in food (such as meat, dairy products and shellfish, etc) and water, and for the detection of GUS gene expression in plants. X-GlcU also has clinical applications in the assessment of urinary tract infection by detecting the presence of E. coli. The substrate yields a dark blue (λmax 615 nm) precipitate after enzymatic hydrolysis.
- White solid soluble in water or DMSO
- Store desiccated at -20°C and protect from light
- 20H26BrClN2O7
- MW: 521.8
- [18656-96-7]
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dense products have been devised. A chromogenic substrate, 5-bromo-4-chloro-3-indolyl phosphate (X-Phos), which is very similar to X-gal, can be employed for detection and leads to production of a blue precipitate (Figure 1). In addition, the tetrazolium
Histochemical detection重组质体的检定
操作原理与colony hybridization 相同，但是将滤膜移置于含有IPTG 及5-bromo-4-chloro-3-indolyl β-D-glucuronide （X-Gluc） 的培养基。 X-Gluc 可被GUS作用而释出蓝色的产物，若转型株所带的质体接有gus 基因的重组质体，则应可在IPTG 的诱导条件下表现出蛋白质，若此重组蛋白质具有GUS 活性，可将进入菌体的X-Gluc 分解而使菌落呈蓝色。 仪器用具： 平端镊子 药品试剂： Nitrocellulose
96-well RNA In Situ Hybridization Protocol
. Wash 5 min at RT with AP buffer. 24. Add 200ul AP buffer containing Nitro Blue Tetrazolium (NBT) and Bromo-Chloro- Indolyl-Phosphatase (BCIP). 25. Incubate with rocking until desired color development is achieved (20 min. to overnight
