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文献和实验affinity for double than single-stranded nucleic acids.Composition of the electrophoresis buffer In the absence of ions, RNA migrates very slowly if at all. If a 10 x buffer is used by mistake, electrical conductance is very efficient, the current
. Composition of the electrophoresis buffer In the absence of ions, RNA migrates very slowly if at all. If a 10 x buffer is used by mistake, electrical conductance is very efficient
, however, it is fine to use regular DNA-style TBE agarose gels. In either case, the RNA should initially be denatured (steps 2-3) and RNAse free reagents should be used. Procedure Add 2 µL RNA (up to 20 µg) to 18 µL 1x Reaction Buffer.
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