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文献和实验and redissolve in 20 µl H 2ODEPC measure RNA concentration and check integrity on a denaturing gel II. cDNA synthesis for each RNA set up four reactions (one tube for each degenerate anchored oligo(dT) primer set - T 12MA,T12 MC
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
sequences from different organisms, or for “evolutionary PCR”, one may increase the chances of getting product by designing “degenerate” primers: these would in fact be a set of primers which have a number of options at several positions in the sequence
of pipetting errors and saves time by reducing the number of reagent transfers.Reaction Mixture Set Up Gently vortex and briefly centrifuge all solutions after thawing. Add, in a thin-walled PCR tube, on ice: Gently vortex the sample and briefly
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