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- AMRESCO
- a-shr-2616
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- CAS号：
553-24-2
- 规格：
25G
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文献和实验Southern Blot using Neutral Transfer
is better for resolving larger fragments. Add ethidium to buffer near red ( ). (0.1 μg/ml ethidium should be included in the gel for visualization). 3) Run gel 5-6 hours at 40 to 60 volts. Once gel is finished running, be sure to take a picture with ruler
. each, RT. Immerse in 4N HCl for 10 min., RT. Immerse in 1x TBE, pH 8.4 for 5 min., RT. Check pH of buffer with pH paper to see if it is neutral. Immerse in 1x PBS. Check pH of buffer after 2 min. Apply primary antibody when pH is 7.0-
.title.x = element_text(colour="red",size=14),axis.text.x = element_text(colour="blue"),axis.title.y = element_text(colour ="red",size=14,angle=90),axis.text.y=element_text(colour="blue"),plot.title=element_text(colour="red",size=20,face="bold
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