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- CAS号:
57-50-1
- 规格:
100ML
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文献和实验Construction and Characterization of Adenovirus Vectors
the cells from the dish into the medium. Transfer to a 4-mL cryovial. x. Add 40% sucrose in PBAd to a final concentration of 4%. Vortex briefly. Use immediately or store at -80°C until use. xi
Procedure for Transfection of Mammalian Cells
in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM
Transformation of Magnaporthe grisea to phosphinothricin resistance
. (1990 Curr. Genet. 17:409-411) was followed. Instead of 1.2 M sorbitol, 20% sucrose was used as osmotic stabilizer. Mycelial pellets from an actively growing, overnight culture were harvested by filtering through a layer of sterile cheesecloth
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