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- 2025年07月13日
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文献和实验8.8 (resolving gel) 1.0 M Tris, pH 6.8 (stacking gel) 5x SDS Running Buffer (1 L) Tris 15 g Glycine 72 g SDS 5 g Coomassie Blue Stain 10% (v/v) acetic acid 0.006% (w/v) Coomassie Blue dye 90% ddH2O Isopropanol Fixing Solution 10
Recombinant expression and SDS
is obtained. 12.The TA will take the gel pictures. SDS -PAGE sample buffer (2X) 0.125 M Tris-CL pH 6.8 4% SDS 20% glycerol 2% 2-mercaptoethanol 0.01% bromphenol blue Acrylamide Solutions for Two Laemmli
from Biorad. Store in fridge.IV. 20% SDS (some people filter)V. 10% w/v ammonium persulfate, make fresh every month and store in fridge. Note: old bottles of dry AP can go bad.VI. N,N,N',N'-tetramethylethylene diamine (TEMED). Buy it and store in fridge.VII
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