HYBRIDIZATION COCKTAIL 45%FORM

Multiplex Fluorescence in situ Hybridization (M-FISH)

Multiplex in situ hybridization (M-FISH) is a 24-color karyotyping technique and is the method of choice for studying complex interchromosomal rearrangements. The process involves three major steps. Firstly, the multiplex labeling

Identification of Bone Marrow Derived Nonhematopoietic Cells by Double Labeling with Immunohistochemistry and In Situ Hybridization

a cocktail of antibodies targeting infiltration inflammatory cells are mandatory. One pitfall of this method is its restriction to sex-mismatched pairs. The spectrum of labels for X and Y chromosmes are suitable for combination. To prevent misinterpretation

荧光原位杂交（FISH）探针的制备及其应用

体异常的识别得益于二十世纪六十年代后发展起来的胰蛋白酶－姬姆萨染色和常规显带技术，使得常规筛查全基因组染色体异常和检测染色体核型改变成为可能。染色体显带是细胞遗传学分析技术中标准和常用的方法，但耗时且依赖于获得良好的分裂相，还难于分析复杂和隐匿的异常。 PCR或荧光原位杂交（FISH，fluorescent in situ hybridization）对染色体异常的检出依赖于引物或探针与模板的结合，因此较常规显带具有更高的特异性，是高通量检测染色体异常的敏感和特异的方法。