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- CAS号：
7791-11-9
- 规格：
50G
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文献和实验Rubidium Chloride method for Transformation Competent E. coli
Rubidium Chloride method for Transformation Competent E. coli Procedure 1. Inoculate 1 ml from overnight culture into 100 ml Psi broth (scale up or down as needed). Incubate at 37 C with aeration to A550=0.48 2. Ice 15 min
This chapter describes a method of assaying rubidium (Rb+ ) efflux as a measure of potassium channel activity. In this assay, rubidium acts as a tracer for potassium movement across the cell membrane. HEK 293 cells expressing the alpha
CONTENT Transformation-Competent E. coli preparation Rubidium chloride method Inoue "ultra-competent" method Cosmid
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