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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20 °C
- 英文名:
BIOTIN-X, SUCCINIMIDYL ESTER (6-((BIOTIN 90051
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
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文献和实验LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
ratio.Dilute 1:40 with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml)/ 43,000} 8.Dilute to 3-5 mg/ml with the dialysis buffer.Calculate total mg of actin.Drop freeze in liquid N2 after dissolving 2 mg sucrose
Labeling Tubulin and Quantifying Labeling Stoichiometry
to tubulin. We have used it successfully to derivatize tubulin with succinimidyl esters of biotin, digoxigenin, and a wide range of fluorochromes such as tetramethylrhodamine, X-rhodamine, fluorescein, Oregon Green, Cy3, Cy5 and C2CF (bis-caged
. The usefulness of the method is shown by the recovery of biotinylated proteins from organ homogenates, obtained from mice perfused with a reactive ester derivative of biotin.












