- 询价
- Biotium
- 美国
- 91036
- 2025年07月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 英文名：
MTS-CR110-X (MTS-5-(AND-6)-CARBOXYRHODAM 91036
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
MTS-CR110-X (MTS-5-(and-6)-carboxyrhodamine 110-X, mixed isomers) is a green fluorescent thiol-reactive dye. It is similar to MTS-CR110 (catalog no. 91035) except that the former has a X spacer molecule between the dye and the MTS reactive group. The linker molecule may enhance fluorescence when the dye is attached to proteins.
- λEx/λEm (MeOH) = 502/524 nm
- Orange solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C30H32N4O7S2
- MW: 624
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文献和实验for gel/blot, aspirate cushion while washing 2-3x with BRB80 and remove as much of the cushion as possible (MTs form a clear, gelatinous pellet). Boil pellet in sample buffer and analyze. MAP and motor pelleting: do not add extra ATP
% glycerol/sucrose cushions. For larger ultra rotors, increase the speed and/or time to reach an equivalent clearing factor. Save supe for gel/blot, aspirate cushion while washing 2-3x with BRB80 and remove as much of the cushion as possible (MTs form
dyneins and central pair MTs from the tail fragments. 17. Transfer the extraction suspension to 50 ml centrifuge tubes and separate the extracted axonemes from soluble proteins by centrifuging them at 20,000 x g (13,000 rpm) for 15 min at 4oC
