- 询价
- Biotium
- 美国
- 92013
- 2025年07月15日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
EOSIN-5-MALEIMIDE 92013
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
Eosin-5-maleimide is a good photosensitizer and can be used to selectively label thiols. It has a quantum yield of 0.57 for singlet oxygen generation, which makes it useful for photoconversion of electron-rich materials such as diaminobenzidine (DAB) or fluorescent dyes into highly electron-dense materials for high resolution electron microscopy studies (1,2). Additionally, eosin is a good fluorescence acceptor for fluorescein (FAM), dansyl and coumarin dyes in fluorescence resonance energy transfer (FRET).
- λEx/λEm (MeOH) = 524/545 nm
- ε = 100,000
- Orange solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C24H9Br4NO7
- MW: 742.95
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文献和实验CY3.18 554 568 CY5.18 649 666 CY7 710 805 DANS (1-DimethylAmino-Naphthaline-5-Sulphonic Acid) 340 525 DANSA(DiaminoNaphthyl-Sulphonic Acid) 340-380 430 Dansyl NH-CH3 in water 340 578 DAPI 350 470 DiA 456 590 Diamino PhenylOxydiazole (DAO) 280
CL-NERF 504(low pH)514(high pH) 587(low pH)540(high pH) Coriphosphine O 460 575 Coumarin-Phalloidin 387 470 CY3.18 554 568 CY5.18 649 666 CY7 710 805 DANS (1-DimethylAmino-Naphthaline-5-Sulphonic Acid) 340 525 DANSA(DiaminoNaphthyl
IN SITU HYBRIDIZATION ON FROZEN SECTIONS
Remove slides from freezer, thaw for 5 min. at 55°C. Fix 10 min. in 4% paraformaldehyde, 4°C. Wash 5 min. in 0.5x SSC, RT. Immerse slides in proteinase K solution , 1-5 µg/ml in RNase Buffer for 10 min., RT
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