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文献和实验the Gel Extraction Spin Protocol. 2) Prepare the vacuum manifold according to manufacturer’ s instruction and connect the V-Spin column to the manifold. 3) Load the dissolved DNA/agarose solution from step 3 to the column.
Materials: agarose solution in TBE or TAE (generally 0.7-1%) 1X TBE or TAE (same buffer as in agarose) gel loading dye
Extraction of DNA from Agarose Gels
by extraction of the DNA fragment of interest from the gel. The importance of this step is obvious from the fact that every vendor of molecular-biology products produces a gel extraction kit. Most of these kits utilize a chaotropic agent, such as sodium iodide
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