Cleaning Solution Refill 4 x60

A protocol for cleaning and reusing the large 25 x 25 cm plates

We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV

Cleaning Worm Stocks

worms, make ~0.6% NaOCl (i.e. 12% vol/vol of the commercial 5% solution), 1.5 M NaOH. Add 50 µl to the unseeded portion of a fresh plate. Place ~10 gravid adults in the drop. Can recover a strain from a single adult if necessary. The adults and bacteria

Protocols: Acid Cleaning Coverslips

  Make-up 300 ml of 2 parts Nitric Acid - 1 part HCl in a glass beaker in the hood. Solution will turn orange-red. Place #1.5 coverslips into solution a few at a time. Let sit for about 2 hrs