凋亡坏死定量试剂盒 (50 assays) 30017

凋亡和坏死是细胞死亡两个主要的过程。凋亡受到细胞内基因调控分解。分解导致细胞膜外侧磷脂含量变化。磷脂酰丝氨酸（PS）从细胞膜内侧翻向外侧。Annexin V 是一段35-36 kDa 的磷脂结合蛋白，与磷脂酰丝氨酸有高度亲 和力。FITC标记的Annexin V通过结合外翻的PS标记凋亡细胞，产生绿色荧光。坏死通常由于细胞严重损伤导致。所有的细胞器和质膜完整性被破坏，导致细胞质溢出，细胞器蛋白进入周围环境中。EtD-III是高带电核酸探针，不能透过活细胞或凋亡细胞的细胞膜，但是能将坏死细胞染成红色。EtD-III所具有的DNA高度亲和力和高荧光量子产量，使其优于PI或ethidium homodimer I，被用于凋亡检测试剂盒中。凋亡、坏死定量试剂盒提供了一个简单方便的检测凋亡细胞（绿色）和坏死细胞（红色）的方法，可用于流式细胞仪或荧光显微镜。

The Apoptosis and Necrosis Quantitation Kit provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

Features

• Simultaneous detection of apoptotic & necrotic cells
• 15-30 minute staining
• EthD-III is a superior alternative to PI and EthD-I

Kit Components

• FITC Annexin V (human; recombinant, produced in E. coli)
• Ethidium Homodimer III (EthD-III)
• 5X Annexin Binding Buffer

Spectral Properties

• FITC Annexin V: Ex/Em 498/517 nm
• EthD-III: Ex/Em 279, 532/625 nm (with DNA)

Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane for phagocytic cell recognition. The human anticoagulant Annexin V is a 35 kDa, Ca²⁺-dependent phospholipid-binding protein with high affinity for PS. Annexin V labeled with fluorescein (FITC) stains apoptotic cells green by binding to PS exposed on the cell surface.

Necrosis normally results from severe cellular insult. Both internal organelle and plasma membrane integrity are lost, resulting in the release of cellular contents into the surrounding environment. Ethidium Homodimer III (EthD-III) is a highly positively charged nucleic acid probe, which is impermeant to live cells, but stains cells with compromised membrane integrity with red fluorescence. EthD-III is a superior alternative to Propidium Iodide (PI) or Ethidium Homodimer I because of its significantly higher affinity for DNA and higher fluorescence quantum yield. The Apoptosis & Necrosis Quantitation Kit provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

Also see the Apoptosis and Necrosis Quantification Kit Plus and Apoptotic, Necrotic, and Healthy Cells Quantification Kit Plus, which feature Annexin V conjugated to Biotium’s superior green fluorescent dye CF®488A, which is brighter and more photostable than FITC.

See our full selection of Cell Viability & Apoptosis Assays and Viability & Apoptosis Assays for Flow Cytometry.