Recombinant Human Leptin
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Recombinant Human Leptin

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  • ¥1080 - 7500
  • PROSPEC
  • 以色列
  • cyt-228
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      Leptin

    • 库存

      部分小规格有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      200ug/1mg/5mg

    规格:200ug产品价格:¥1080.0
    规格:1mg产品价格:¥2415.0
    规格:5mg产品价格:¥7500.0

    Recombinant Human Leptin

    CATALOGUE NUMBER

    CYT-228

    SYNONYMS

    OB Protein, Obesity Protein, OBS, Obesity factor.

    INTRODUCTION

    A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

    DESCRIPTION

    Leptin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16 kDa.
    The Leptin is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

    SOLUBILITY

    The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.

    STABILITY

    Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C.

    PURITY

    Greater than 95.0% as determined by:
    (a) Analysis by SEC-HPLC.
    (b) Analysis by SDS-PAGE.

    AMINO ACID SEQUENCE

    The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.

    BIOLOGICAL ACTIVITY

    Biological Activity is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

    PROTEIN CONTENT

    Protein quantitation was carried out by two independent methods:
    1. UV spectroscopy at 280 nm using the absorbency value of 0.878 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
    2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard.

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    SAFETY DATA SHEET

    SDS

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    图标文献和实验
    该产品被引用文献

    1.Title:Leptin and Amylin Act in an Additive Manner to Activate Overlapping Signaling Pathways in Peripheral Tissues In vitro and ex vivo studies in humans.
    Publication:Published online before print September 24, 2010, doi: 10.2337/dc10-0518 Diabetes Care January 2011 vol. 34 no. 1 132-138 .


    2.Title:The Mammalian Target of Rapamycin as Novel Central Regulator of Puberty Onset via Modulation of Hypothalamic Kiss1 System.
    Publication: Published online before print September 4, 2009, doi: 10.1210/en.2009-0096 Endocrinology November 1, 2009 vol. 150 no. 11 5016-5026

    3.Title:p38 Mitogen-Activated Protein Kinase and Liver X Receptor-? Mediate the Leptin Effect on Sterol Regulatory Element Binding Protein-1c Expression in Hepatic Stellate Cells.
    Publication:Mol Med. 2012; 18(1): 10–18.

    4. Title: PI3K integrates the effects of and leptin on large-conductance Ca2+-activated K+ channels in neuropeptide Y neurons of the hypothalamic arcuate nucleus

    Publication:  American Journal of Physiology-Endocrinology and Metabolism 298.2 (2010): E193-E201.

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    文献支持
    Recombinant Human Leptin
    ¥1080 - 7500