Recombinant Pufferfish Leptin产品图
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Recombinant Pufferfish Leptin

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  • ￥1600 - 30429
  • PROSPEC
  • 以色列
  • cyt-530
  • 2025年12月26日
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    上海经科化学科技有限公司
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    • 文献和实验
    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      pfLeptin

    • 库存：

      部分小规格有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      20ug/100ug/1mg

    规格：20ug产品价格：￥1600.0
    规格：100ug产品价格：￥5000.0
    规格：1mg产品价格：￥30429.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-530

    SYNONYMS

    OB Protein, Obesity Protein, OBS, Obesity factor.

    INTRODUCTION

    A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

    DESCRIPTION

    Leptin Pufferfish (Takifugu rubripes) Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 16 kDa.
    Bioactive Leptin Pufferfish (Takifugu rubripes) Recombinant was prepared according to the sequence published by Kurokawa et al. (2005)Peptides 26, 745-750 in two forms: monomer and covalent dimer. MS analysis revealed molecular masses of 15,291 and 30,585 Da, close to the theoretical values of 15,270 and 30,540 Da. CD spectra revealed high similarity to mammalian leptins. Other details of its preparation will be soon published by Yacobovitz et al (in press), General and Comparative Endocrinology.
    The Pufferfish Leptin is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    The Pufferfish Leptin was lyophilized from a concentrated (0.85mg/ml) solution with 0.003mM NaHCO3.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Pufferfish Leptin in sterile 0.4% NaHCO3 pH-9 not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Pufferfish Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 99.0% as determined by:
    (a) Analysis by SEC-HPLC.
    (b) Analysis by SDS-PAGE.

    AMINO ACID SEQUENCE

    ALPGALDAMDVEKMKSKVTWKAQGLVARIDKHFPDRGLRFDTDKVE

    GSTSVVASLESYNNLISDRFGGVSQIKTEISSLAGYLNHWREGNCQE

    QQPKVWPRRNIFNHTVSLEALMRVREFLKLLQKNVDLLERC

    PROTEIN CONTENT

    Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

    BIOLOGICAL ACTIVITY

    Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The affinity of human leptin receptors is considerably lower campared to mammalian leptins.

    SAFETY DATA SHEET

    SDS

    USAGE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    该产品被引用文献

    A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

    相关实验

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      01  靶点：FGF21 应用：肥胖、2型糖尿病等代谢疾病的潜在治疗靶点来源：FGF21 synergizes with leptin to counteract obessity related metabolic comorbidities by reversing hepatic  leptin resistance.Cell Metab,2026 Sep 1     香港大学徐爱民团队近期在Cell Metabolism发表了一篇研究，开发了长效FGF21与瘦素双

    • 【第98期】前沿靶点速递 | LAG3、FGF21、HSF1等9项重磅研究进展

      靶点：FGF21     应用：肥胖、2型糖尿病及MASLD等代谢疾病的潜在治疗靶点           来源：FGF21 synergizes with leptin to counteract obesity-related metabolic comorbidities by reversing hepatic leptin resistance.Cell Metab, 2026 Sep 1   香港大学徐爱民教授团队近期在Cell Metabolism发表了一篇研究，首次

    • AAV-Mediated Gene Transfer to Skeletal Muscle

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    Recombinant Pufferfish Leptin
    ￥1600 - 30429