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Recombinant Human Leptin Tripl

e Antagonist
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  • ¥1080 - 26000
  • PROSPEC
  • 以色列
  • cyt-352
  • 2025年12月26日
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 保存条件

      for future use below -18°C

    • 保质期

      See instructions

    • 英文名

      Leptin tA

    • 库存

      部分小规格有备货

    • 供应商

      上海经科化学科技有限公司

    • CAS号

    • 规格

      10ug/50ug/1mg

    规格:10ug产品价格:¥1080.0
    规格:50ug产品价格:¥2415.0
    规格:1mg产品价格:¥26000.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-352

    DESCRIPTION

    Leptin Antagonist Triple Mutant Human Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular weight of 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A. Leptin Antagonist Triple Mutant Human Recombinant was purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia coli.

    PHYSICAL APPEARANCE

    White lyophilized (freeze-dried) powder.

    FORMULATION

    The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Human Recombinant in sterile 0.4% NaHCO3, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Leptin Antagonist Triple Mutant Human Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 mg/ml and up to 2 mg/ml and filter sterilization Leptin mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested.
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 98.0% as determined by:
    (a) Gel filtration analysis.
    (b) Analysis by SDS-PAGE.

    AMINO ACID SEQUENCE

    The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.

    BIOLOGICAL ACTIVITY

    ProSpec’s Leptin triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.

    PROTEIN CONTENT

    Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

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    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    该产品被引用文献

    Title: Leptin-induced vascular smooth muscle cell proliferation via regulating cell cycle, activating ERK1/2 and NF-kB
    Publication: Acta biochimica et biophysica Sinica 42.5 (2010): 325-331.

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    文献支持
    Recombinant Human Leptin Triple Antagonist
    ¥1080 - 26000