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Recombinant Human Epidermal Gr

owth Factor
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  • ￥1080 - 3864
  • PROSPEC
  • 以色列
  • cyt-217
  • 2026年02月05日
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    上海经科化学科技有限公司
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    • 技术资料
    • 保存条件：

      for future use below -18°C

    • 保质期：

      See instructions

    • 英文名：

      EGF

    • 库存：

      部分小规格有备货

    • 供应商：

      上海经科化学科技有限公司

    • CAS号：

      无

    • 规格：

      100ug/0.5mg/1mg

    规格：100ug产品价格：￥1080.0
    规格：0.5mg产品价格：￥2415.0
    规格：1mg产品价格：￥3864.0

    产品细节图片1

    CATALOGUE NUMBER

    CYT-217

    SYNONYMS

    Urogastrone, URG, EGF.

    INTRODUCTION

    Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

    DESCRIPTION

    Epidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6.2kDa. The EGF is purified by proprietary chromatographic techniques.

    SOURCE

    Escherichia Coli.

    PHYSICAL APPEARANCE

    Sterile Filtered White lyophilized (freeze-dried) powder.

    FORMULATION

    EGF was lyophilized from a concentrated (1mg/ml) solution containing PBS pH-7.4.

    SOLUBILITY

    It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    STABILITY

    Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.

    PURITY

    Greater than 98.0% as determined by SDS-PAGE.

    AMINO ACID SEQUENCE

    NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR.

    BIOLOGICAL ACTIVITY

    The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.

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    该产品被引用文献

    Activation of the Hedgehog pathway in pilocytic astrocytomas.
    Publication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010

     
    EGR-1 activation by EGF inhibits MMP-9 expression and lymphoma growth

    Publication:   Blood 116.5 (2010): 759-766.

     
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    文献支持
    Recombinant Human Epidermal Growth Factor
    ￥1080 - 3864