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- 2025年07月10日
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文献和实验In an effort to produce renm mhibttory peptides, the preparation of (2 S ,4 S ,5 S )-5-( t -Butoxycarbonylamino)-4-( t -butyldimethylsilyloxy)-2-isopropyl-7-methyl octanotc acid, 15 , was carried out ( 1 ). This pseudo-dipeptide was used
Protocol for Trichloroacetic Acid (TCA) Precipitation
Introduction The efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replication) can be determined by trichloroacetic acid (TCA) precipitation. TCA
Phosphoamino acid analysis:Mark Kamps method
Phosphoamino acid analysis:Mark Kamps's method 1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P .
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